Exploring the Identity and Properties of Two Bacilli Strains and their Potential to Alleviate Drought and Heavy Metal Stress

Naturally available plant growth-promoting rhizobacteria (PGPR) have 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes, and are capable of processing the plant-borne ACC by converting it into α-ketobutyrate ammonia. Thus, PGPRs help in depletion ethylene levels, enhance abiotic stress tolerance plants. In present study, two rhizobacterial strains, i.e., Bacillus cereus B. haynesii, isolated from Vigna mungo Phaseolus vulgaris, were used. These strains taxonomically identified 16S rDNA sequencing as with NCBI accession numbers LC514122 LC 514123, respectively. The phylogeny these has also been worked out based on homology, data GenBank. screened for their traits, quantified same way. enzymatic activity molecular weight obtained both bacterial determined. An vitro drought study was done using PEG 6000. exhibited higher (~5 to 6 µmol/mL), exopolysaccharide yield (15 18 mg/10 mL protein), indole acetic (27–32 µg/mL). characteristics indicate that under may be helpful enhancing crops enhanced yield. found a tolerant strain As, Ba, Ni, plate assay method, so potential used biofertilizer fields affected metals.